HPLC Systems Laborgeräte > Gebrauchte & günstige HPLC Systems [en]

18127

Hewlett Packard 1090L
Hewlett Packard HPLC system 1090 L. Isocratic system. 1 x low-pressure pump. Column oven. Autosampler for max. 100 Vials. DAD 79880 . He-Degasser. 230 V. 50 Hz. Max. 750 W. HPIB interface. PC. Software HPLC 3D ChemStation (DOS series) G1307A, Rev. A. 02.02. Operating fiield. Display.

Product group: HPLC Systems

Stock unit The device is in our stock, however, has to be inspected by our technicien, in order to be able to guarantee complete function. Therefore the delivery time is generally four to six weeks.

The device is in our stock, however, has to be inspected by our technicien, in order to be able to guarantee complete function. Therefore the delivery time is generally four to six weeks.

Column for free

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Price: 3,500.00 €
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17282

Waters HPLC
Waters HPLC system. Autosampler 717 Plus. 220 V. Tray for 48 vials. CE-marked. IEEE interface. Column oven PCO 200. With control unit. 220 V. 50 Hz. Automated valve station Wavs. Programmable multiwave length detector 490 E. 2x Shimadzu double piston pump LC-10 AD. Perkin Elmer Interface NCI 900. HP Computer Vectra VE. Monitor. Printer LaserJet 4L.

Product group: HPLC Systems

Stock unit The device is in our stock, however, has to be inspected by our technicien, in order to be able to guarantee complete function. Therefore the delivery time is generally four to six weeks.

Column for free

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Price: 7,800.00 €
(excluding VAT)

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17222

Dionex HPLC/AD20
Dionex HPLC-system. Detector AD20. 190-800 nm. Flowcell: 10 mm. PEEK. Max. 1000 psi. Double-beam. 220 V. 50 Hz. Firmware 3.02. Isocrated pump IP20. Double piston. Max. 350 bar. 0,04-10 mL/min. 220 V. 50 Hz. DX-Lan-interface. Digitale indication. Operating field. CE-marked.Universal interface UI20. Merck-Hitachi LaChrom detector L7490. 220 V. 50 Hz. 60 W. CE-marked. Rheodyne 6-way-valve serie 7000 with 20µL sample loop

Product group: HPLC Systems

Stock unit The device is in our stock, however, has to be inspected by our technicien, in order to be able to guarantee complete function. Therefore the delivery time is generally four to six weeks.

Column for free

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Price: 7,400.00 €
(excluding VAT)

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11107

Waters HPLC 3
Waters HPLC-System. With gradient pump 600 with controller 600 E. Firmware 3.0, 1988. Waters autosampler 715 Ultra WISP. Tray for 48 positions. Programmable PDA-detector 991. NEC SX Plus computer with 5,25\"drive, monitor and keyboard. Printer/plotter 5200. Software 820 on 5,25\" floppy disks.

Product group: HPLC Systems

Stock unit The device is in our stock, however, has to be inspected by our technicien, in order to be able to guarantee complete function. Therefore the delivery time is generally four to six weeks.

Column for free

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Price: 4,100.00 €
(excluding VAT)

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HPLC Systems

HPLC is a physical method to separate, identify and quantify substances by distribution between the mobile, moving and the stationary, steady phase.

Functionality of HPLC

HPLC, high performance liquid chromatography, is a separation process where the liquid sample is carried by the liquid phase through the separation column over the stationary phase, on account of high pressure. Length and diameter of the separation column vary according to application. In some cases, a precolumn is placed before the HPLC column to remove impurities before separation. In contrast to gas chromatography, no gaseous or non-decomposed evaporateable substances are necessary for HPLC. In the case of strong interaction of the analyte with the stationary phase, the analyte remains in the column for quite a long time. If there is weak interaction, the opposite case is true: The analyte exits the column quite quickly. Therefore the substances appear at the end of the separation column after different times, the so-called characteristic retention times. HPLC can be differentiated by two principles: normal phase and reversal phase chromatography. In the case of NP-HPLC, a polar stationary phase is used, for example, silicon gel. In this process, the polarity determines the elution force of the mobile phase. The solvents are getting arranged by rising polarity, resulting in the so-called eluotropic series. This means that substances with higher polarity elute faster. Polar molecules better interact with the polar stationary phase and remain therefore longer in the column. RP-HPLC applies a non-polar stationary phase. The elution force decreases with rising polarity.

Classification of HPLC

In HPLC, several separation mechanisms can be distinguished, depending on the kind of interaction between stationary phase, mobile phase and the sample: adsorption, partition, ion exchange, size exclusion and bioaffinity chromatography. However, adsorption and partition chromatography are the most common modes of HPLC.

Architecture of an HPLC system

An HPLC system consists of an eluent vessel, a degasser, a pump, an injection valve, a precolumn, a column, a detector and an evaluation unit.

Literature

http://de.wikipedia.org/w/index.php?title=Hochleistungsfl%C3%BCssigkeitschromatographie&oldid=86523806 (called: 30.03.11).